Resveratrol stimulates microRNA expression during differentiation of bovine primary myoblasts

Background: Resveratrol (RSV), a phenolic compound, is present in many human dietary sources, such as peanuts, peanut butter, grapes skin, and grape wine. RSV has been widely known for its benefits on health. Beef from cattle skeletal muscle one of the main sources protein consumption. Previous studies have also found that pork chicken qualities are influenced by feed supplementation with RSV. In addition, our previous study demonstrated effects bovine myoblast differentiation using messenger RNA (mRNA) data. this study, we mainly focused influences microRNA (miRNA) expression. Method: We used 20 μM to treat primary myoblasts extracted miRNA sequencing. After quality control alignment clean reads, conducted quantification analysis differentially expressed (DE) miRNAs case (RSV-treated) group versus (non-RSV treated) group. Next, predicted target genes DE analyzed them enrichments Gene Ontology (GO) terms Kyoto Encyclopedia Genes Genomes (KEGG) pathways. Results: Finally, identified 93 (adjusted P-value < 0.05), 44 were upregulated 49 downregulated. Bta-miR-34c was most significantly miRNA. silico, prediction results indicated 1,869 miRNAs. GO enrichment targeted revealed two significant which stereocilium (GO:0032420). KEGG showed five pathways, top pathway insulin signaling (bta04910) 0.05). Conclusions: This provided an improved understanding through modulations. The suggested could promote stimulating expressions. enriched pathway, thus potentially contributing improving leanness increasing energy metabolism.